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| Registros recuperados: 12 | |
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| Popovic,Tatjana; Milovanovic,Predrag; Aleksic,Goran; Gavrilovic,Veljko; Starovic,Mira; Vasic,Mirjana; Balaž,Jelica. |
| Halo blight, caused by Pseudomonas savastanoi pv. phaseolicola (Psp), is considered to be an important bacterial disease on common bean (Phaseolus vulgaris L.) in Serbia. Use of pathogen-free seeds is one of the most effective control measures against this disease. The aim of this study was to evaluate a detection method for Psp on untreated common bean seeds (23 genotypes) from commercial crops grown within Serbia. Detection of this pathogen was made by plating onto the modified sucrose peptone (MSP) and Milk Tween (MT) semi-selective mediums from soaked whole common bean seed. Colonies growing on the MSP medium were light yellow, convex and shiny, whereas on the MT medium, they were creamy white, flat and circular. The pathogenicity of the obtained... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MSP; MT; ELISA; Nested-PCR; Halo blight; Pathogenicity. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000400005 |
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| Cezarino,Bruno Nicolino; Yamamoto,Lidia; Del Negro,Gilda Maria Barbaro; Rocha,Daisy; Okay,Thelma Suely. |
| Group B streptococcus (GBS) remains the most common cause of early-onset sepsis in newborns. Laboratory gold-standard, broth culture methods are highly specific, but lack sensitivity. The aim of this study was to validate a nested-PCR and to determine whether residue volumes of urine samples obtained by non invasive, non sterile methods could be used to confirm neonatal GBS sepsis. The nested-PCR was performed with primers of the major GBS surface antigen. Unavailability of biological samples to perform life supporting exams, as well as others to elucidate the etiology of infections is a frequent problem concerning newborn patients. Nevertheless, we decided to include cases according to strict criteria: newborns had to present with signs and symptoms... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: GBS; Neonatal sepsis; Early-onset neonatal sepsis; Neonatal infection; PCR; Nested-PCR. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100005 |
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| Fonseca Júnior,Antônio Augusto; Carmagos,Marcelo Fernandes; D'Ambros,Régia Maria Feltrin; Braga,Alexandre Carvalho; Ciacci-Zanella,Janice; Heinemann,Marcos Bryana; Leite,Rômulo Cerqueira; Reis,Jenner Karlisson Pimenta dos. |
| A pseudoraiva (PR) é uma enfermidade viral responsável por consideráveis perdas econômicas na indústria de suínos. O vírus da pseudoraiva (PrV) apresenta apenas um sorotipo, mas, por análise de restrição enzimática, foi classificado em quatro genótipos denominados I, II, III e IV. Os métodos usados para genotipagem dependem do isolamento do vírus, da purificação do DNA viral, da restrição enzimática do genoma completo e da visualização após eletroforese. O objetivo deste trabalho foi estabelecer um método mais rápido e sensível para detectar e genotipar o PrV por nested-PCR e análise de restrição enzimática. Vinte isolados do PrV das regiões Sul e Sudeste do Brasil e a estirpe padrão Shope foram replicadas em células PK-15 e submetidas à nested-PCR para o... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genotipagem; Nested-PCR; Pseudoraiva; Restrição enzimática. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000400027 |
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| Araújo,Cristina P.; Osório,Ana Luiza A.R.; Jorge,Klaudia S.G.; Ramos,Carlos A.N.; Souza Filho,Antonio F.; Vidal,Carlos E.S.; Vargas,Agueda P.C.; Roxo,Eliana; Rocha,Adalgiza S.; Suffys,Philip N.; Fonseca Júnior,Antônio A.; Silva,Marcio R.; Barbosa Neto,José D.; Cerqueira,Valíria D.; Araújo,Flábio R.. |
| Post-mortem bacterial culture and specific biochemical tests are currently performed to characterize the etiologic agent of bovine tuberculosis. Cultures take up to 90 days to develop. A diagnosis by molecular tests such as PCR can provide fast and reliable results while significantly decreasing the time of confirmation. In the present study, a nested-PCR system, targeting rv2807, with conventional PCR followed by real-time PCR, was developed to detect Mycobacterium tuberculosis complex (MTC) organisms directly from bovine and bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other Actinomycetales species and DNA samples... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine and bubaline tuberculosis; Nested-PCR; Real-time PCR; Tissue; Sanitary inspection. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200035 |
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| Cruz,Heidi Lacerda Alves da; Montenegro,Rosana de Albuquerque; Lima,Juliana Falcão de Araújo; Poroca,Diogo da Rocha; Lima,Juliana Figueirêdo da Costa; Montenegro,Lílian Maria Lapa; Crovella,Sergio; Schindler,Haiana Charifker. |
| The polymerase chain reaction (PCR) and its variations, such as the nested-PCR, have been described as promising techniques for rapid diagnosis of tuberculosis (TB). With the aim of evaluating the usefulness of a nested-PCR method on samples of blood and urine of patients suspected of tuberculosis we analyzed 192 clinical samples, using as a molecular target the insertion element IS6110 specific of M. tuberculosis genome. Nested-PCR method showed higher sensitivity in patients with extrapulmonary tuberculosis (47.8% and 52% in blood and urine) when compared to patients with the pulmonary form of the disease (sensitivity of 29% and 26.9% in blood and urine), regardless of the type of biological sample used. The nested-PCR is a rapid technique that, even if... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacterium tuberculosis; Nested-PCR; Blood; Urine; Molecular diagnosis; IS6110 insertion sequence. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100041 |
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| Zucatto,A.S.; Aquino,M.C.C.; Inácio,S.V.; Figueiredo,R.N.; Pierucci,J.C.; Perri,S.H.V.; Meireles,M.V.; Bresciani,K.D.S.. |
| Considering the proximity of sheep farmers to animals that are possibly diseased or releasing fecal oocysts into the environment and the marked pathogenicity in lambs, the aim of this study was to determine the occurrence and to molecularly characterize the infection by Cryptosporidium spp. in lambs in the South Central region of the state of São Paulo, Brazil. A total of 193 fecal samples were collected from sheep of several breeds, males and females, aged up to one year. Polymerase chain reaction (nested-PCR) was used to amplify DNA fragments from the subunit 18S rRNA gene and indicated 15% positivity; sequencing of amplified fragments was possible for 19 samples. Analysis of the obtained sequences showed that the identified species were Cryptosporidium... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cryptosporidiosis; Sheep; Nested-PCR; Genotype. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000200441 |
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| Mello Junior,Carlos Cesar de; Delsol,Gael Yvan Leclercq; Motte,Emmerik; Escobar,Virna Alexia Cedeño; Rey,Pedro Filipe; Martins,Mauricio Laterça; Arana,Luis Alejandro Vinatea; Mello,Giovanni Lemos de; Farias,Alvaro Pestana de; Arguello,Xavier Antonio Serrano; Maridueña,John Erick Montaño. |
| The objective of this work was to select surviving breeders of Litopenaeus vannamei from white spot syndrome virus (WSSV) outbreak, adapted to local climatic conditions and negatively diagnosed for WSSV and infectious hypodermal and hematopoietic necrosis virus (IHHNV), and to evaluate if this strategy is a viable alternative for production in Santa Catarina, Brazil. A total of 800 males and 800 females were phenotypically selected in a farm pond. Nested-PCR analyses of 487 sexually mature females and 231 sexually mature males showed that 63% of the females and 55% of the males were infected with IHHNV. Animals free of IHHNV were tested for WSSV, and those considered double negative were used for breeding. The post-larvae produced were stocked in nine... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Litopenaeus vannamei; Breeding; Epidemiology; Nested-PCR. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000500011 |
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| Chacón,JLV; Brandão,PEB; Villarreal,LYB; Gama,NM; Ferreira,AJP. |
| Trachea, lung, and conjunctive samples from 51 commercial layer farms from Bastos region, São Paulo, Brazil, were submitted to nested-PCR and virus isolation in SPF chicken embryos for ILT diagnosis. This region experienced an outbreak characterized by respiratory signs, decrease in egg production and increased mortality. Out of the 51 tested field samples, 23 were positive for ILT by nested-PCR, 22 were positive after the virus isolation, and 24 were positive when both techniques were used. Newcastle disease virus, Avian pneumovirus, or Mycoplasma gallisepticum were not detected. Infectious bronchitis virus was detected in one farm and Mycoplasma synoviae was detected in eight farms. The high incidence of infectious laryngotracheitis virus (ILTV)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Infectious laryngotracheitis; Nested-PCR; Virus isolation. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2007000100009 |
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| Rios,R.L.; Marin,S.Y.; Gomes,A.M.; Resende,J.S.; Bonfim,M.R.Q.; Gomes,A.D.; Resende,F.M.S.; Horta,R.S.; Resende,M.; Martins,N.R.S.. |
| Fifty-four fecal samples taken from broiler chickens from 1 to 45 days of age, and of pullets from 10 to 13 weeks of age, original from eight different poultry regions in the state of Minas Gerais, Brazil, were collected from March 2008 to January 2010 for avian Orthoreovirus (ARV) and avian Rotavirus (AvRV) analyses. For the assay of ARV, RNA was immediately extracted (Trizolâ) and transcribed into cDNA for assaying in a nested-PCR with ARV-specific primers. For AvRV, polyacrylamide gel electrophoresis (PAGE) was performed with RNA extracts obtained by phenol-chloroform extraction. CAV was additionally investigated through a nested-PCR of thymus and spleen. Results found 5.55% positive for ARV and 9.25% for AvRV. Also, CAV and ARV genomes were detected in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broiler; Layer; Chicken anemia virus; CAV; Avian reovirus; ARV; Avian rotavirus; AvRV; Nested-PCR; PAGE. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000600030 |
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| Registros recuperados: 12 | |
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